Raimondas MOZURAITIS-1,2, Anna-Karin BORG-KARLSON-1,
Alvaro EIRAS-3. Peter WITZGALL-4, Adalécio KOVALESKI-5,
Evaldo F. VILELA-6, C. Rikard UNELIUS-1
1- The Royal institute of Technology, Department of Chemistry,
Organic Chemistry, S-10044 Stockholm,SWEDEN
2- Institute of Ecology, Section of Chemical Ecology,
Academijos 2, Vilnius, LITHUANIA.
3- Setor de Semioquimicos, Laboratorio de Pratecao de Plantas, CCTA,
Universidade Estadual do Norte Fluminense UENF), Campos, Rt1, 28045-620,
BRAZIL
4- Swedish University of Agricultural Sciences, Department of
Chemical Ecology S230 53 Alnarp, SWEDEN
5- Departamento de Entomologia, EMBRA/CNPUV, Vacaria, RS, 25200,
BRAZIL
6- Departamento de Biologia Animal, Universidade Federal de Vicosa,
Vicosa, MG,36570, BRAZIL.
The SPME (solid phase micro extraction) technique was used in the collection of volatiles emitted by caJing females of the leaFroller moth Bonagota cranaodes (Lepidoptera, Tortricidae). The main compound (>95% of total amounts) released by the calling B. cranaodes females were identified by GC-MS as the (3E,5Z)-3,5dodecadienyl acetate. This biologically active acetate was found only in trace to minor amounts in the heptane extracts of the abdominal glands. The amount of volatiles released by one calling female during three hours collected on a polydimethylsiloxane fibre, was as large as the amount extracted from the glands of 60 females. The SPME technique gives the opportunity of continuously following the release of behaviour mediated signals from weak scented living organisms.
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